SB-HPLC provides a rapid and efficient method for the identification of potential immunomodulatory components in traditional Chinese medicines. It is suggested that PAN contributes to the anti-DTH effects of YPFS.
In vivo, PAN was found to attenuate allergic contact dermatitis in a mouse model of delayed-type hypersensitivity (DTH), a pharmacological activity not previously reported for this molecule. Bioassay in vitro demonstrated that PAN significantly inhibited splenocyte proliferation induced by concanavalin A (ConA) in a concentration-dependent manner, whereas LA had no significant effect on splenocyte proliferation. You can easily control and monitor instruments by connecting them to a LAN on which the ChemStation PC resides.
This allowed identification of the molecule as panaxynol (PAN) based on EI-MS and NMR spectrometry. The ChemStation software provides LAN-based instrument control and data acquisition for the Agilent 7890 GC, the Agilent 6890 GC, the Agilent 35900E A/D control module and the Agilent 1100/1200 Series LC. Silica gel chromatography was used to purify compound C1 from Radix Saposhnikoviae, a major constituent of YPFS. Compound C2 was identified as linoleic acid (LA) based on UV absorption and mass spectrometry.
It works with both single spectrum files and multi spectrum files.
After enrichment by splenocyte binding, two YPFS components (C1 and C2) were analyzed by HPLC. Hi folks, the Spectragryph optical spectroscopy software is now able to open HP/ Agilent 8453. To screen for bioactive components we combined splenocyte binding with high performance liquid chromatography (SB-HPLC). Yu-ping-feng-san (YPFS) is a widely used immunomodulatory herbal medication used in traditional Chinese medicine, but the active molecules remain obscure.